Genetic Detection of IMP-1 Gene and its Relationship with Biofilm Formation in Klebsiella pneumonia

Background: Klebsiella pneumoniae were considered as normal flora of skin, and intestine. It can cause damage to human lungs; the danger this bacterium is related exposure hospital surroundings.
 materials methods: detection on morphological biochemical tests then assured with VITEK 2 system. Resistance antibiotics was determined by Kirby-Baeur method. And genotyping IMP-1 in isolates done PCR technique, biofilm formation identified Micro titer plate method.
 Results: The present study included a collecting 50 specimens from different clinical specimens, (blood 40%, urine 30%, sputum 20%, wound infection 10%); 10 .
 All isolates, under study, developed high resistance toward Cefitrixon, Ampicillin, Amoxicillin, Ticarcillin, Ticarcillin+Clavulanic acid, Ceftazidim estimated disc diffusion characterized harboring highest resistant percentage reached 100% against antibiotics, study. This Minimal Inhibitory Concentration detected eight E-test strips for isolates. As well strong production three while moderate other weak former; at value (P≤0.05) significant. Genotype Metalo-beta lactamase (IMP-1) technique . Upon using exposed only isolates;30% (two urine, one blood) samples harbored gene. also found relationship between which these genes, when (P ≤ 0.05).
 Conclusions: K. isolated sources. most used have Metallo-beta lactamse. showed gene,.This there gene (P≤0.05).